The escalating threat of poaching and illegal trade in wildlife internationally has its roots in social inequalities, organized crime, and demand from consumers. The poaching and trafficking of wildlife endanger species and facilitate greater biodiversity loss aggravated by habitat destruction and environmental degradation. With many species hanging by a thread, we desperately need effective tools for investigation in wildlife crime. Forensics, especially advances in forensic genetics like DNA profiling and stable isotope analysis, are of great importance in the investigation of wildlife crimes. These give the capacity for authorities to trace the origin of seized wildlife products, identify poached animals, and connect offenders with these crimes, thereby strengthening the legal case and resulting in successful prosecution. This article reviews the development and utilization of forensic techniques for wildlife crimes, how scientific evidence has undermined international trafficking networks, and notable case studies that show how these innovations have been put into enforcement strategies for justice. These efforts support the goals set forth by the United Nations. It aids biodiversity conservation (SDG 15-Life on Land); enhances legal enforcement (SDG 16-Peace, Justice, and Strong Institutions); and discourages illegal exploitation of wildlife (SDG 12-Responsible Consumption and Production). The intersection of forensic science with conservation and law enforcement activities serves the dual purpose of combating wildlife crime and advancing pillars of global mark on sustainability, environmental justice, and the protection of vulnerable ecosystems.