DNA profiling has attained the role of being an established part of the criminal justice process with high admissibility rates in the courtroom. With the advancements in science and technology, it has acted as a promoter in the shift in scientific innovation, ranging from health, economics, and security; to trade, sustainability and justice. Locard’s Exchange Principle based on the fact that every contact leaves a trace offers a standpoint to DNA profiling based on the promise of 99% certainty and reliability. Unfortunately, the scientific promise of DNA has dark sides in its application to courtrooms. With a backdrop to DNA processes like PCR, RFLP, CAE and MCE; the paper analyzes the DNA transfer stages and profile matching techniques - Comparison, Random Match Probability and the Likelihood Ratio.1 Dwelling into the hierarchy of propositions, the paper offers an insight into the 3 models of DNA interpretation: Legal, Liberative and Scientific. From the legal perspective, the International and National legislative backing is discussed in lines with contested rights of fair trial, presumption of innocence, privacy, integrity and autonomy. This research unraveled the cynicism of DNA profiling by diving into the issues of the CSI effect and the landmark Castro case. Issues of contamination, inaccurate results, error rates, probability statistics, interpretation standards quality samples, examiners’ bias, laboratory slope and the Hardy-Weinberg equation is assessed.2 Miscarriage of courts to uphold the Frye and Relevancy Test are deciphered with an aim to showcase the problem of the ‘infallibility proposition.3Taking from the experience and lessons from judicial pronouncements, the paper concludes with forensic and legal recommendations to uphold the probative and evidentiary value of DNA profiling.